Lecia Epping
Lecia Epping

Research Assistant III 
lecia-pewe@uiowa.edu  

Department of Pathology
25 S. Grand Ave, 1020 ML
University of Iowa 
Iowa City, IA 52242

Lab: 319-335-9919

Training

B.S. (Biology) Saint Ambrose University, Davenport, IA

Research

Design and develop experiments to determine the localization and distribution of CD8 T-cell memory cells during infections in splenic and liver tissues by confocal microscopy and multi-photon intravital imaging. 

Publications Pubmed

  1. Lefebvre MN, Surette FA, Anthony SM, Vijay R, Jensen IJ, Pewe LL, Hancox LS, Van Braeckel-Budimir N, van de Wall S, Urban SL, Mix MR, Kurup SP, Badovinac VP, Butler NS, Harty JT. Expeditious recruitment of circulating memory CD8 T cells to the liver facilitates control of malaria. Cell Rep. 2021 Nov 2;37(5):109956. doi: 10.1016/j.celrep.2021.109956. PMID: 34731605; PMCID: PMC8628427.
  2. Lefebvre MN, Drewry LL, Pewe LL, Hancox LS, Reyes-Sandoval A, Harty JT. Cutting Edge: Subunit Booster Vaccination Confers Sterilizing Immunity against Liver-Stage Malaria in Mice Initially Primed with a Weight-Normalized Dose of Radiation-Attenuated Sporozoites. J Immunol. 2021 Dec 1;207(11):2631-2635. doi: 10.4049/jimmunol.2100818. Epub 2021 Oct 29. PMID: 34716185; PMCID: PMC8612975.
  3. Kurup SP, Moioffer SJ, Pewe LL, Harty JT. p53 Hinders CRISPR/Cas9-Mediated Targeted Gene Disruption in Memory CD8 T Cells In Vivo. J Immunol. 2020 Oct 15;205(8):2222-2230. doi: 10.4049/jimmunol.2000654. Epub 2020 Sep 4. PMID: 32887747; PMCID: PMC7541733.
  4. Urban SL, Jensen IJ, Shan Q, Pewe LL, Xue HH, Badovinac VP, Harty JT. Peripherally induced brain tissue-resident memory CD8+ T cells mediate protection against CNS infection. Nat Immunol. 2020 Aug;21(8):938-949. doi: 10.1038/s41590-020-0711-8. Epub 2020 Jun 22. PMID: 32572242; PMCID: PMC7381383.
  5. Brate AA, Boyden AW, Itani FR, Pewe LL, Harty JT, Karandikar NJ. Therapeutic intervention in relapsing autoimmune demyelinating disease through induction of myelin-specific regulatory CD8 T cell responses. J Transl Autoimmun. 2019 Dec;2:100010. doi: 10.1016/j.jtauto.2019.100010. PMID: 32161909; PMCID: PMC7065686.
  6. de Greef JC, Slütter B, Anderson ME, Hamlyn R, O'Campo Landa R, McNutt EJ, Hara Y, Pewe LL, Venzke D, Matsumura K, Saito F, Harty JT, Campbell KP. Protective role for the N-terminal domain of α-dystroglycan in Influenza A virus proliferation. Proc Natl Acad Sci U S A. 2019 Jun 4;116(23):11396-11401. doi: 10.1073/pnas.1904493116. Epub 2019 May 16. PMID: 31097590; PMCID: PMC6561248.
  7. Kurup SP, Anthony SM, Hancox LS, Vijay R, Pewe LL, Moioffer SJ, Sompallae R, Janse CJ, Khan SM, Harty JT. Monocyte-Derived CD11c+ Cells Acquire Plasmodium from Hepatocytes to Prime CD8 T Cell Immunity to Liver-Stage Malaria. Cell Host Microbe. 2019 Apr 10;25(4):565-577.e6. doi: 10.1016/j.chom.2019.02.014. Epub 2019 Mar 21. PMID: 30905437; PMCID: PMC6459714.
  8. Schmidt ME, Knudson CJ, Hartwig SM, Pewe LL, Meyerholz DK, Langlois RA, Harty JT, Varga SM. Memory CD8 T cells mediate severe immunopathology following respiratory syncytial virus infection. PLoS Pathog. 2018 Jan 2;14(1):e1006810. doi: 10.1371/journal.ppat.1006810. PMID: 29293660; PMCID: PMC5766251.
  9. Itani FR, Sinha S, Brate AA, Pewe LL, Gibson-Corley KN, Harty JT, Karandikar NJ. Suppression of autoimmune demyelinating disease by preferential stimulation of CNS-specific CD8 T cells using Listeria-encoded neuroantigen. Sci Rep. 2017 May 8;7(1):1519. doi: 10.1038/s41598-017-01771-8. PMID: 28484224; PMCID: PMC5431563.
  10. Doll KL, Pewe LL, Kurup SP, Harty JT. Correction: Discriminating Protective from Nonprotective Plasmodium-Specific CD8+ T Cell Responses. J Immunol. 2016 Aug 1;197(3):1003. doi: 10.4049/jimmunol.1600905. Erratum for: J Immunol. 2016 May 15;196 (10 ):4253-62. PMID: 27431985.
  11. Li Y, Kinzenbaw DA, Modrick ML, Pewe LL, Faraci FM. Context-dependent effects of SOCS3 in angiotensin II-induced vascular dysfunction and hypertension in mice: mechanisms and role of bone marrow-derived cells. Am J Physiol Heart Circ Physiol. 2016 Jul 1;311(1):H146-56. doi: 10.1152/ajpheart.00204.2016. Epub 2016 Apr 22. PMID: 27106041; PMCID: PMC4967211.
  12. Doll KL, Pewe LL, Kurup SP, Harty JT. Discriminating Protective from Nonprotective Plasmodium-Specific CD8+ T Cell Responses. J Immunol. 2016 May 15;196(10):4253-62. doi: 10.4049/jimmunol.1600155. Epub 2016 Apr 15. Erratum in: J Immunol. 2016 Aug 1;197(3):1003. PMID: 27084099; PMCID: PMC4868661.
  13. Richer MJ, Pewe LL, Hancox LS, Hartwig SM, Varga SM, Harty JT. Inflammatory IL-15 is required for optimal memory T cell responses. J Clin Invest. 2015 Sep;125(9):3477-90. doi: 10.1172/JCI81261. Epub 2015 Aug 4. PMID: 26241055; PMCID: PMC4588296.
  14. Slütter B, Pewe LL, Kaech SM, Harty JT. Lung Airway-Surveilling CXCR3(hi) Memory CD8(+) T Cells Are Critical for Protection against Influenza A Virus. Immunity. 2013 Nov 14;39(5):939-48. PubMed PMID: 24238342. 
  15. Cabrera M, Pewe LL, Harty JT, Frevert U. In vivo CD8+ T Cell Dynamics in the Liver of Plasmodium yoelii Immunized and Infected Mice. PLoS One. 2013;8(8): e70842. doi: 10.1371/journal.pone.0070842.
  16. Nolz JC, Harty JT. Protective capacity of memory CD8+ T cells is dictated by antigen exposure history and nature of the infection. Immunity. 2011;34(5):781-793. doi:10.1016/j.immuni.2011.03.020
  17. Schmidt NW, Butler NS, Harty JT. Plasmodium-host interactions directly influence the threshold of memory CD8 T cells required for protective immunity. J Immunol. 2011;186(10):5873-5884. doi:10.4049/jimmunol.1100194
  18. Butler NS, Schmidt NW, Vaughan AM, Aly AS, Kappe SH, Harty JT. Superior antimalarial immunity after vaccination with late liver stage-arresting genetically attenuated parasites. Cell Host Microbe. 2011;9(6):451-462. doi:10.1016/j.chom.2011.05.008
  19. Butler NS, Moebius J, Pewe LL, Traore B, Doumbo OK, Tygrett LT, Waldschmidt TJ, Crompton PD, Harty JT. Therapeutic blockade of PD-L1 and LAG-3 rapidly clears established blood-stage Plasmodium infection. Nat Immunol. 2011 Dec 11;13(2):188-95. doi: 10.1038/ni.2180.
  20. Kashiwada M, Pham NL, Pewe LL, Harty JT, Rothman PB. NFIL3/E4BP4 is a key transcription factor for CD8α+ dendritic cell development. Blood. 2011 Jun 9;117(23):6193-7. Epub 2011 Apr 7.
  21. Khanolkar A, Fulton RB, Epping LL, Pham NL, Tifrea D, Varga SM, Harty JT. T cell epitope specificity and pathogenesis of mouse hepatitis virus-1-induced disease in susceptible and resistant hosts. J Immunol. 2010 Jul 15;185(2):1132-41.
  22. Pham NL, Pewe LL, Fleenor CJ, Langlois RA, Legge KL, Badovinac VP, Harty JT. Exploiting cross-priming to generate protective CD8 T-cell immunity rapidly. Proc Natl Acad Sci U S A. 2010 Jul 6;107(27):12198-203.
  23. Khanolkar A, Hartwig SM, Haag BA, Meyerholz DK, Epping LL, Haring JS, Varga SM, Harty JT. Protective and pathologic roles of the immune response to mouse hepatitis virus type 1: implications for severe acute respiratory syndrome. J Virol. 2009 Sep;83(18):9258-72.

Publication acknowledgements of technical assistance:

  1. Anthony SM, Van Braeckel-Budimir N, Moioffer SJ, et al. Protective function and durability of mouse lymph node-resident memory CD8+ T cells. Elife. 2021;10:e68662. Published 2021 Jun 18. doi:10.7554/eLife.68662
  2. Martin MD, Jensen IJ, Ishizuka AS, et al. Bystander responses impact accurate detection of murine and human antigen-specific CD8 T cells. J Clin Invest. 2019;129(9):3894-3908. Published 2019 Jun 20. doi:10.1172/JCI124443
  3. Danahy DB, Kurup SP, Winborn CS, et al. Sepsis-Induced State of Immunoparalysis Is Defined by Diminished CD8 T Cell-Mediated Antitumor Immunity. J Immunol. 2019;203(3):725-735. doi:10.4049/jimmunol.1900435
  4. Danahy DB, Jensen IJ, Griffith TS, Badovinac VP. Cutting Edge: Polymicrobial Sepsis Has the Capacity to Reinvigorate Tumor-Infiltrating CD8 T Cells and Prolong Host Survival. J Immunol. 2019;202(10):2843-2848. doi:10.4049/jimmunol.1900076
  5. Van Braeckel-Budimir N, Varga SM, Badovinac VP, Harty JT. Repeated Antigen Exposure Extends the Durability of Influenza-Specific Lung-Resident Memory CD8+ T Cells and Heterosubtypic Immunity. Cell Rep. 2018;24(13):3374-3382.e3. doi:10.1016/j.celrep.2018.08.073
  6. Kurup SP, Obeng-Adjei N, Anthony SM, et al. Regulatory T cells impede acute and long-term immunity to blood-stage malaria through CTLA-4. Nat Med. 2017;23(10):1220-1225. doi:10.1038/nm.4395
  7. Strother RK, Danahy DB, Kotov DI, et al. Polymicrobial Sepsis Diminishes Dendritic Cell Numbers and Function Directly Contributing to Impaired Primary CD8 T Cell Responses In Vivo. J Immunol. 2016;197(11):4301-4311. doi:10.4049/jimmunol.1601463
  8. Condotta SA, Khan SH, Rai D, Griffith TS, Badovinac VP. Polymicrobial Sepsis Increases Susceptibility to Chronic Viral Infection and Exacerbates CD8+ T Cell Exhaustion. J Immunol. 2015;195(1):116-125. doi:10.4049/jimmunol.1402473
  9. Khanolkar A, Williams MA, Harty JT. Antigen experience shapes phenotype and function of memory Th1 cells. PLoS One. 2013;8(6):e65234. Published 2013 Jun 7. doi:10.1371/journal.pone.0065234
  10. Richer MJ, Nolz JC, Harty JT. Pathogen-specific inflammatory milieux tune the antigen sensitivity of CD8(+) T cells by enhancing T cell receptor signaling. Immunity. 2013;38(1):140-152. doi:10.1016/j.immuni.2012.09.017
  11. Schmidt NW, Khanolkar A, Hancox L, Heusel JW, Harty JT. Perforin plays an unexpected role in regulating T-cell contraction during prolonged Listeria monocytogenes infection. Eur J Immunol. 2012;42(3):629-640. doi:10.1002/eji.201141902
  12. Schmidt NW, Harty JT. Cutting Edge: Attrition of Plasmodium-Specific Memory CD8 T Cells Results in Decreased Protection That Is Rescued by Booster Immunization. J Immunol. 2011 Feb 28. 
  13. Butler NS, Schmidt NW, Harty JT. Differential effector pathways regulate memory CD8 T cell immunity against Plasmodium berghei versus P. yoelii sporozoites. J Immunol. 2010 Mar 1;184(5):2528-38. Epub 2010 Jan 22.
  14. Zhao DM, Yu S, Zhou X, Haring JS, Held W, Badovinac VP, Harty JT, Xue HH. Constitutive activation of Wnt signaling favors generation of memory CD8 T cells. J Immunol. 2010 Feb 1;184(3):1191-9. Epub 2009 Dec 21.
  15. Schmidt NW, Butler NS, Harty JT. CD8 T cell immunity to Plasmodium permits generation of protective antibodies after repeated sporozoite challenge. Vaccine. 2009 Oct 19;27(44):6103-6. Epub 2009 Aug 25.